Metastatic esophagogastric cancer treatments after failure of second-line chemotherapy are limited. Nivolumab demonstrated superior overall survival (OS) versus placebo in Asian patients with advanced gastric or gastroesophageal junction cancers. We assessed the safety and efficacy of nivolumab and nivolumab plus ipilimumab in Western patients with chemotherapy-refractory esophagogastric cancers.

Patients with locally advanced or metastatic chemotherapy–refractory gastric, esophageal, or gastroesophageal junction cancer from centers in the United States and Europe received nivolumab or nivolumab plus ipilimumab. The primary end point was objective response rate. The association of tumor programmed death-ligand 1 status with response and survival was also evaluated.

Of 160 treated patients (59 with nivolumab 3 mg/kg, 49 with nivolumab 1 mg/kg plus ipilimumab 3 mg/kg, 52 with nivolumab 3 mg/kg plus ipilimumab 1 mg/kg), 79% had received two or more prior therapies. At the data cutoff, investigator-assessed objective response rates were 12% (95% CI, 5% to 23%), 24% (95% CI, 13% to 39%), and 8% (95% CI, 2% to 19%) in the three groups, respectively. Responses were observed regardless of tumor programmed death-ligand 1 status. With a median follow-up of 28, 24, and 22 months across the three groups, 12-month progression-free survival rates were 8%, 17%, and 10%, respectively; 12-month OS rates were 39%, 35%, and 24%, respectively. Treatment-related grade 3/4 adverse events were reported in 17%, 47%, and 27% of patients in the three groups, respectively.

Nivolumab and nivolumab plus ipilimumab demonstrated clinically meaningful antitumor activity, durable responses, encouraging long-term OS, and a manageable safety profile in patients with chemotherapy-refractory esophagogastric cancer. Phase III studies evaluating nivolumab or nivolumab plus ipilimumab in earlier lines of therapy for esophagogastric cancers are underway.

Metastatic esophagogastric cancer is a global health burden and a substantial cause of cancer-related mortality worldwide.1,2 For patients with disease progression receiving second-line therapy, the prognosis remains poor; thus, effective treatment options are urgently needed.3,4 The pathogenesis of esophagogastric adenocarcinoma has been linked to chronic inflammation, DNA damage that results in high microsatellite instability (MSI), high mutational burden, and overexpression of immune checkpoint proteins.5-8 These findings suggest that immune checkpoint inhibition is a viable therapeutic strategy for patients with esophagogastric cancer. The anti–programmed death-1 (PD-1) monoclonal antibodies nivolumab and pembrolizumab have demonstrated promising activity in early clinical trials that included patients with esophagogastric cancers.9-11

On the basis of superior survival demonstrated in the phase III, randomized, placebo-controlled ATTRACTION-2 trial,12 nivolumab was approved in Japan for the treatment of patients with chemotherapy-refractory gastric and gastroesophageal junction (GEJ) cancers regardless of programmed death-ligand 1 (PD-L1) status. Also, in the United States, pembrolizumab was approved for the treatment of patients with chemotherapy-refractory PD-L1–positive gastric/GEJ cancer on the basis of the promising clinical activity observed in the KEYNOTE-059 trial.10 Dual PD-1/cytotoxic T-lymphocyte–associated antigen 4 blockade with nivolumab plus ipilimumab has demonstrated synergistic activity in preclinical models13,14 and has led to enhanced response rates in patients with metastatic melanoma, small-cell lung cancer, and DNA mismatch repair–deficient/MSI–high (MSI-H) metastatic colorectal cancer.15-17 We present the safety, efficacy, long-term survival, and biomarker analyses of nivolumab and nivolumab plus ipilimumab in Western patients with chemotherapy-refractory locally advanced or metastatic esophagogastric cancer from the multicenter, phase I/II CheckMate-032 trial.

Study Design and Treatment

CheckMate-032 is an ongoing, open-label, two-stage, multicohort, phase I/II trial. The esophagogastric cohort of CheckMate-032 enrolled patients at 18 centers in the United States and five European countries. The study protocol and all amendments were approved by local institutional review boards, and the protocol was conducted in accordance with the Declaration of Helsinki and Good Clinical Practice Guidelines, as defined by the International Conference on Harmonisation of Technical Requirements for Pharmaceuticals for Human Use. All patients provided written informed consent before enrollment. Patients were randomly assigned, when multiple treatment groups were open, to one of the following: nivolumab 3 mg/kg (NIVO3) intravenously every 2 weeks; nivolumab 1 mg/kg plus ipilimumab 3 mg/kg (NIVO1 + IPI3) every 3 weeks for four cycles; or nivolumab 3 mg/kg plus ipilimumab 1 mg/kg (NIVO3 + IPI1) every 3 weeks for four cycles. All combination regimens were followed by NIVO3 every 2 weeks until disease progression or unacceptable adverse event (AE). Treatment beyond disease progression was permitted in patients with clinical benefit on the basis of investigator assessment. Patients who were assigned to the NIVO3 group and experienced disease progression could cross over to a combination group. Dose reductions or modifications were not permitted with nivolumab or ipilimumab. Dose interruption was allowed. The criteria for treatment discontinuation and interruption are summarized in the Appendix (online only).


Key eligibility criteria for the esophagogastric cancer cohort included diagnosis of locally advanced or metastatic gastric, esophageal, or GEJ adenocarcinoma with disease progression while taking or intolerance of at least one chemotherapy regimen; measurable disease as assessed by Response Evaluation Criteria in Solid Tumors (RECIST) version 1.118; Eastern Cooperative Oncology Group performance status of 0 or 1; and adequate organ function. Patients with human epidermal growth factor receptor 2–positive tumors were eligible if they had received previous treatment with trastuzumab. Key exclusion criteria included suspected autoimmune disease; hepatitis B virus or human immunodeficiency virus infection; conditions requiring corticosteroids or other immunosuppressive medications; and previous immune checkpoint inhibitor therapy.

Study Assessments

The primary end point was objective response rate (ORR), defined as the best response of complete response or partial response divided by the number of treated patients, per RECIST version 1.1.18 ORR was assessed by investigator and by blinded independent central review (BICR). Secondary end points included overall survival (OS), progression-free survival (PFS), duration of response (DOR), and safety. Tumor response was assessed using imaging every 6 weeks for 24 weeks, then every 12 weeks until disease progression or treatment discontinuation. Survival was monitored continuously while patients were receiving treatment and every 3 months after treatment discontinuation. Clinical activity was also assessed by tumor PD-L1 and MSI status. AEs were assessed and graded using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.0.19

Tumor PD-L1 expression was assessed centrally using a validated, automated immunohistochemistry assay (Dako North America, Carpinteria, CA) of archival samples obtained before enrollment or new biopsy specimens. Samples with ≥ 100 evaluable tumor cells and ≥ 1% PD-L1 staining of tumor cell membranes were considered PD-L1–positive. MSI status was established retrospectively on available tumor/normal paired samples using a polymerase chain reaction–based assay on the basis of the Bethesda panel of mononucleotide and dinucleotide markers.20 Samples positive for two or more markers of instability were classified as MSI-H.

Statistical Analyses

Each treatment group was evaluated separately for the primary end point of ORR using a modified Simon two-stage study design,21 with 80% power to reject the null hypothesis of ORR ≤ 10% (insufficient drug activity), assuming that the true ORR was 25%, with a one-sided α of .05. Thus, the trial was not designed or powered for a formal comparison of the treatment groups. In this multicohort trial, an ORR of 25% was considered to be of clinical interest across tumor types and was not specifically selected based on the esophagogastric cancer cohort. In addition, the tests did not adjust for multiplicity across the different tumor type cohorts in CheckMate-032. In the first stage, 18 patients were enrolled per group. If at least two responses were observed, accrual was expanded in the second stage to 22 additional patients, for a total of 40 patients per group. The treatment was considered of clinical interest if at least 20% of patients (eight of 40) experienced an objective response. On the basis of the results of phase I studies in other solid tumors,22-24 it was hypothesized that NIVO3 + IPI1 would have similar efficacy and improved safety; thus, the NIVO3 + IPI1 group was not based on a two-stage design and was started once the NIVO1 + IPI3 group proceeded to the second stage. The protocol permitted further expansion of treatment groups on the basis of the clinical activity.

The BICR assessment of the NIVO3 group was based on the March 2016 data cutoff. All other efficacy and safety assessments for the treatment groups were based on the November 2016 data cutoff. ORR was summarized by a binomial proportion and corresponding two-sided 95% exact CIs using the Clopper-Pearson method. DOR, PFS, and OS were summarized using medians and time point–specific survival rates by Kaplan-Meier and two-sided 95% CIs. Descriptive statistics were used to characterize patient characteristics and safety. Statistical analyses were performed using SAS software (version 9.2; SAS Institute, Cary, NC).

Patient Characteristics

Patients were enrolled and treated from November 19, 2013, through June 3, 2015. At the time of the data cutoff, 160 patients received NIVO3 (n = 59), NIVO1 + IPI3 (n = 49), or NIVO3 + IPI1 (n = 52). Of the 160 patients, 79% had received two or more prior therapies; 49%, 47%, and 38% of patients across the three groups had received three or more prior lines of therapy, respectively (Table 1). Tumor samples were evaluable for PD-L1 and MSI status in 79% and 45% of patients, respectively. The NIVO3 group had higher percentages of patients with PD-L1–positive tumors (38%) and MSI-H status (28%) than either of the combination groups (NIVO1 + IPI3: PD-L1–positive, 24%, and MSI-H, 9%; NIVO3 + IPI1: PD-L1–positive, 30%, and MSI-H, 8%). Median duration of follow-up (potential time on study from first dose to database lock) in the three groups was 28 months (range, 17 to 35 months) in the NIVO3 group, 24 months (range, 21 to 33 months) in the NIVO1 + IPI3 group, and 22 months (range, 19 to 25 months) in the NIVO3 + IPI1 group; most patients in each treatment group (NIVO3, 97%; NIVO1 + IPI3, 88%; NIVO3 + IPI1, 94%) had discontinued treatment at the time of the data cutoff. The most common reason for discontinuation of treatment across all groups was disease progression (Appendix Fig A1, online only). After discontinuation of study therapy, approximately one third of patients in each treatment group (36% overall) went on to receive subsequent anticancer therapy, consisting of chemotherapy in most patients (84%).


Table 1. Baseline Patient and Disease Characteristics


Investigator-assessed ORR was 12% with NIVO3, 24% with NIVO1 + IPI3, and 8% with NIVO3 + IPI1 (Table 2). Median DOR was 7.1 months (95% CI, 3.0 to 13.2 months) in the NIVO3 group, 7.9 months (95% CI, 2.8 months to not estimable) in the NIVO1 + IPI3 group, and not yet reached (95% CI, 2.5 months to not estimable) in the NIVO3 + IPI1 group (Figs 1A-1C). Responses were observed with NIVO3, NIVO1 + IPI3, and NIVO3 + IPI1 regardless of tumor PD-L1 expression (Table 3; BICR in Appendix Table A1, online only). Objective responses were observed in both patients with MSI-H and those with non–MSI-H tumors (Table 3; Appendix Fig A2, online only). Additional efficacy outcomes per BICR are presented in Appendix Figure A3 (online only).


Table 2. ORR, DCR, and DOR per Investigator Assessment and BICR


Table 3. Investigator-Assessed Response and OS by PD-L1 and MSI Status

Among evaluable patients, 29%, 45%, and 27% of patients in the NIVO3, NIVO1 + IPI3, and NIVO3 + IPI1 groups, respectively, had a reduction in tumor burden from baseline per investigator assessment (Fig 1D; BICR in Appendix Fig A4, online only). The median time to response ranged from 1.6 to 2.7 months (Table 2). Disease control (complete response, partial response, and stable disease) was achieved by 32% to 41% of patients (Table 2). Stable disease for at least 12 weeks was achieved by 67%, 63%, and 67% of patients with stable disease in the NIVO3, NIVO1 + IPI3, and NIVO3 + IPI1 groups, respectively. At the time of the data cutoff, five patients in the NIVO1 + IPI3 group and two patients in the NIVO3 + IPI1 group had ongoing responses by investigator assessment (Appendix Fig A2, online only).

Median PFS by investigator assessment was 1.4 months (95% CI, 1.2 to 1.5 months) in the NIVO3 group, 1.4 months (95% CI, 1.2 to 3.8 months) in the NIVO1 + IPI3 group, and 1.6 months (95% CI, 1.4 to 2.6 months) in the NIVO3 + IPI1 group (Fig 2A; BICR in Appendix Fig A5, online only). The 12-month PFS rate was 8% (95% CI, 3% to 17%) in the NIVO3 group, 17% (95% CI, 8% to 29%) in the NIVO1 + IPI3 group, and 10% (95% CI, 3% to 20%) in the NIVO3 + IPI1 group. The median OS was 6.2 months (95% CI, 3.4 to 12.4 months) in the NIVO3 group, 6.9 months (95% CI, 3.7 to 11.5 months) in the NIVO1 + IPI3 group, and 4.8 months (95% CI, 3.0 to 8.4 months) in the NIVO3 + IPI1 group (Fig 2B). The 12-month OS rate was 39% (95% CI, 26% to 52%) in the NIVO3 group, 35% (95% CI, 22% to 49%) in the NIVO1 + IPI3 group, and 24% (95% CI, 13% to 37%) in the NIVO3 + IPI1 group. The 12-month OS rates by PD-L1 and MSI status are listed in Table 3.


Treatment-related AEs (TRAEs) occurred in 69% of patients in the NIVO3 group, 84% of patients in the NIVO1 + IPI3 group, and 75% of patients in the NIVO3 + IPI1 group. The most common (≥ 15%) TRAEs across all treatment groups included fatigue, pruritus, rash, diarrhea, decreased appetite, and increased ALT and AST levels (Table 4). Grade 3/4 TRAEs were reported in 17%, 47%, and 27% of patients receiving NIVO3, NIVO1 + IPI3, and NIVO3 + IPI1, respectively. TRAEs resulted in treatment discontinuation in 3% of patients in the NIVO3 group, 20% of patients in the NIVO1 + IPI3 group, and 13% of patients in the NIVO3 + IPI1 group. Serious TRAEs occurred in 10%, 43%, and 25% of patients receiving NIVO3, NIVO1 + IPI3, and NIVO3 + IPI1, respectively. One death due to tumor lysis syndrome, deemed by the investigator to be possibly treatment related, occurred in the NIVO3 + IPI1 group.


Table 4. Treatment-Related Adverse Events

Results of the CheckMate-032 study reported here demonstrate for the first time that nivolumab and nivolumab plus ipilimumab provide clinically meaningful and durable antitumor activity with a manageable safety profile in heavily pretreated Western patients with chemotherapy-refractory esophagogastric cancer. Notably, the clinical activity with nivolumab monotherapy in our study was consistent with that reported with nivolumab in Asian patients in the ATTRACTION-2 study.12 Taken together with other reports on anti–PD-1 therapy,9,25 these findings suggest that despite the morphologic and molecular heterogeneity of esophagogastric cancer, immune checkpoint blockade provides a consistent therapeutic benefit across Asian and Western patients.

Considering the aggressive biology of metastatic esophagogastric cancer, combined immune checkpoint blockade may further improve the efficacy of single-agent anti–PD-1 therapy by avoiding tumor immune escape through synergistic T-cell antitumor activity.13,14 NIVO1 + IPI3 has demonstrated clinical activity and a manageable safety profile in other solid tumors15,16 and is Food and Drug Administration approved for the treatment of melanoma.26 The results with NIVO1 + IPI3 therapy reported here demonstrate an ORR of 24%; however, despite the numerically higher ORR achieved in patients receiving NIVO1 + IPI3 than in those receiving NIVO3, median OS was similar between these groups. One explanation for this observation may be the higher proportion of patients with MSI-H and PD-L1–positive tumors in the NIVO3 group. The enhanced clinical benefit observed with NIVO1 + IPI3 was accompanied by a numerically higher incidence of grade 3/4 AEs than observed with NIVO3. These events were primarily diarrhea and elevated liver enzyme levels and were manageable using protocol-specified AE management algorithms. In contrast, NIVO3 + IPI1 had comparable clinical activity and a numerically higher overall rate of AEs compared with NIVO3. These findings suggest that the lower ipilimumab dose may not have been sufficient to enhance anti–PD-1–mediated immune responses in this patient population. On the basis of the numerically higher overall response and landmark OS rates in the NIVO1 + IPI3 arm, this combination was considered more likely to offer clinical benefit relative to currently available treatment regimens for first-line metastatic esophagogastric cancer and was selected for further evaluation in the phase III CheckMate-649 study (NCT02872116).

To identify potential biomarkers of response to nivolumab and nivolumab plus ipilimumab, treatment response and outcomes were explored by tumor PD-L1 and MSI status. Responses were observed regardless of tumor PD-L1 status across the treatment groups. Although the ORR seemed numerically higher in patients with PD-L1–positive versus PD-L1–negative tumors, the sample size was small, with overlapping CIs between these subgroups. Of note, tumor PD-L1 status was not predictive of survival with nivolumab in patients with gastric/GEJ cancer in the phase III ATTRACTION-2 trial.12

With emerging data highlighting the importance of MSI as a predictive biomarker of response to immune checkpoint inhibitors,27,28 our exploratory analysis revealed that responses were observed in patients with both MSI-H and non–MSI-H tumors. The ORR seemed numerically higher in the MSI-H subgroup; however, because of the small sample size, these data are only hypothesis generating, and research in larger patient subsets is needed to confirm these findings.

This study adds to the current body of evidence supporting the role of immune checkpoint inhibitors for the treatment of patients with advanced esophagogastric cancers.9,10,12 Limitations of this phase I/II study include the absence of a standard-of-care comparator and that the study was not designed for formal comparisons across treatment groups. In addition, identification of potential biomarkers of response was limited by the small sample size. Ongoing studies may identify biomarker-defined subgroups of patients likely to gain greater benefit from nivolumab-based therapy. Thus, the optimal approach of when (earlier v later lines of therapy) and how (alone or in combination) to incorporate nivolumab and nivolumab plus ipilimumab into clinical practice is yet to be determined.

In summary, our findings suggest that nivolumab and nivolumab plus ipilimumab represent a potential therapeutic approach for patients with advanced esophagogastric cancer. Ongoing phase III studies are investigating nivolumab in the adjuvant setting (NCT02743494) and NIVO1 + IPI3 in the first-line setting (NCT02872116) in patients with esophagogastric cancer.

© 2018 by American Society of Clinical Oncology

Supported by Bristol-Myers Squibb, Princeton, NJ.

Presented in part at the 2016 ASCO Annual Meeting, Chicago, IL, June 3-7, 2016; the 2017 ASCO Annual Meeting, Chicago, IL, June 2-6, 2017; and the 2017 Annual Meeting of the European Society for Medical Oncology, Madrid, Spain, September 8-12, 2017.

Clinical trial information: NCT01928394.

Conception and design: Yelena Y. Janjigian, Marina Tschaika, Dung T. Le

Provision of study materials or patients: Emiliano Calvo, Katriina Peltola, Dung T. Le

Collection and assembly of data: Yelena Y. Janjigian, Johanna Bendell, Emiliano Calvo, Joseph W. Kim, Paolo A. Ascierto, Padmanee Sharma, Patrick A. Ott, Katriina Peltola, Dirk Jaeger, Jeffry Evans, Filippo de Braud, Ian Chau, Dung T. Le

Data analysis and interpretation: All authors

Manuscript writing: All authors

Final approval of manuscript: All authors

Accountable for all aspects of the work: All authors

CheckMate-032 Study: Efficacy and Safety of Nivolumab and Nivolumab Plus Ipilimumab in Patients with Metastatic Esophagogastric Cancer

The following represents disclosure information provided by authors of this manuscript. All relationships are considered compensated. Relationships are self-held unless noted. I = Immediate Family Member, Inst = My Institution. Relationships may not relate to the subject matter of this manuscript. For more information about ASCO's conflict of interest policy, please refer to or

Yelena Y. Janjigian

Consulting or Advisory Role: Eli Lilly, Bristol-Myers Squibb, Merck, Roche/Genentech

Research Funding: Boehringer Ingelheim, Merck, Bristol-Myers Squibb

Johanna Bendell

Research Funding: Eli Lilly (Inst), Genentech/Roche (Inst), Incyte (Inst), Gilead Sciences (Inst), Bristol-Myers Squibb (Inst), Leap Therapeutics (Inst), AstraZeneca/MedImmune (Inst), Boston Biomedical (Inst), GlaxoSmithKline (Inst), Novartis (Inst), Array BioPharma (Inst), Taiho Pharmaceutical (Inst), Celgene (Inst), OncoMed (Inst), Oncogenex (Inst), Daiichi Sankyo (Inst), Bayer (Inst), Apexigen (Inst), Kolltan Pharmaceuticals (Inst), SynDevRx (Inst), Merck (Inst), Macrogenics (Inst), Five Prime Therapeutics (Inst), EMD Serono (Inst), TG Therapeutics (Inst), Boehringer Ingelheim (Inst), Forty Seven (Inst), Stem CentRx (Inst), Onyx (Inst), Sanofi (Inst), Takeda (Inst), Abbott/AbbVie (Inst), Eisai (Inst), Celldex (Inst), Agios (Inst), ARMO BioSciences (Inst), CytomX Therapeutics (Inst), Nektar (Inst), Ipsen (Inst), Merrimack (Inst), Novartis (Inst), Tarveda (Inst), Tyrogenex (Inst), Marshall Edwards (Inst), Pieris Pharmaceuticals (Inst), Mersana (Inst), Calithera Biosciences (Inst), Blueprint Medicines (Inst), Gritstone Oncology (Inst), Evelo Therapeutics (Inst), FORMA Therapeutics (Inst), Merus (Inst), Jacobio (Inst), eFFECTOR Therapeutics (Inst)

Emiliano Calvo

Employment: START, HM Hospitales Group

Leadership: START

Stock and Other Ownership Interests: START, Oncoart Associated, International Cancer Consultants

Honoraria: HM Hospitales Group

Consulting or Advisory Role: Novartis, Janssen-Cilag, PsiOxus Therapeutics, Seattle Genetics, EUSA Pharma, Abbvie, Celgene, AstraZeneca, Guidepoint Global, GLG, SERVIER, Amcure, Roche/Genentech, Nanobiotix, Pfizer

Speakers' Bureau: Novartis

Research Funding: Novartis, AstraZeneca, BeiGene, START

Travel, Accommodations, Expenses: Roche/Genentech

Other Relationship: President and Founder of Foundation INTHEOS (Investigational Therapeutics in Oncological Sciences)

Joseph W. Kim

Consulting or Advisory Role: Voluntis, TRM Oncology, AstraZeneca/MedImmune, Clovis Oncology

Research Funding: Immune Design

Paolo A. Ascierto

Consulting or Advisory Role: Bristol-Myers Squibb, Roche/Genentech, Merck Sharp & Dohme, Novartis, Amgen, Array BioPharma, Merck Serono, Pierre Fabre, Newlink Genetics, Genmab, Incyte, MedImmune, AstraZeneca, Syndax

Research Funding: Bristol-Myers Squibb (Inst), Roche/Genentech (Inst), Array BioPharma (Inst)

Padmanee Sharma

Stock or Other Ownership: Jounce Therapeutics, Neon Therapeutics, Jounce Therapeutics (I), Neon Therapeutics (I), Constellation Pharmaceuticals, Oncolytics, BioAtla, Forty Seven, Apricity Therapeutics, Polaris, Marker Therapeutics, Codiak Biosciences, BioAtla (I), Forty Seven (I), Apricity (I), Polaris (I), Marker Therapeutics (I), Codiak Biosciences (I)

Consulting or Advisory Role: Constellation Pharmaceuticals, Jounce Therapeutics, Kite Pharma, Neon Therapeutics, BioAtla, Pieris Pharmaceuticals, Oncolytics Biotech, Merck, BioMx, Forty Seven, Polaris, Apricity Therapeutics, Marker Therapeutics, Codiak Biosciences, Jounce Therapeutics (I), Kite Pharma (I), Neon Therapeutics (I), Amgen, Apricity Therapeutics (I), Polaris (I), Marker Therapeutics (I), Codiak Biosciences (I)

Patents, Royalties, Other Intellectual Property: Own patent licensed to Jounce Therapeutics, own patents licensed to Bristol-Myers Squibb (I), Jounce Therapeutics (I), Merck (I)

Patrick A. Ott

Consulting or Advisory Role: Bristol-Myers Squibb, CytomX Therapeutics, Celldex, Genentech, Neon Therapeutics, Novartis, Pfizer, Merck

Research Funding: Bristol-Myers Squibb (Inst), Merck (Inst), AstraZeneca/MedImmune (Inst), Celldex (Inst), ARMO BioSciences (Inst)

Katriina Peltola

Employment: Orion Pharma

Stock or Other Ownership: Faron Pharmaceuticals

Consulting or Advisory Role: MSD Oncology, Eli Lilly, Orion Pharma, Pfizer, Bristol-Myers Squibb, Novartis, Roche, Ipsen Travel, Accommodations, Expenses: Bristol-Myers Squibb

Dirk Jaeger

Consulting or Advisory Role: Bristol-Myers Squibb, Roche, Genentech, Bayer, CureVac, Novartis, Amgen

Jeffry Evans

Consulting or Advisory Role: Bristol-Myers Squibb (Inst), Karus Therapeutics (Inst), Eisai (Inst), Immunova (Inst)

Speakers' Bureau: Bristol-Myers Squibb, Eisai

Research Funding: AstraZeneca (Inst), Celgene (Inst), Basilea (Inst), Eisai (Inst), Merck (Inst), Daiichi Sankyo (Inst), Verastem (Inst), Immunocore (Inst), TC Biopharm (Inst), Roche/Genentech (Inst), GlaxoSmithKline (Inst)

Travel, Accommodations, Expenses: Bristol-Myers Squibb, Eisai

Filippo de Braud

Consulting or Advisory Role: Ignyta, Pfizer, Amgen, Novartis, Daiichi Sankyo, Bristol-Myers Squibb, Servier, Celgene, Tiziana Life Sciences, Dompé

Speakers' Bureau: MSD, Novartis, Bristol-Myers Squibb, Roche, Menarini, Pfizer, Servier

Research Funding: Novartis (Inst), Ignyta (Inst), MedImmune (Inst), Nektar, Bristol-Myers Squibb (Inst), Bayer (Inst), Eli Lilly (Inst)

Travel, Accommodations, Expenses: Roche, Amgen, Bristol-Myers Squibb, Daiichi Sankyo

Ian Chau

Honoraria: Taiho Pharmaceutical, Pfizer, Eli Lilly, Amgen, Gilead Sciences

Consulting or Advisory Role: Sanofi, Eli Lilly, Bristol-Myers Squibb, MSD Oncology, Bayer, Roche/Genentech, Five Prime Therapeutics, Merck Serono

Research Funding: Janssen-Cilag (Inst), Sanofi (Inst), Merck Serono (Inst), Eli Lilly (Inst)

Travel, Accommodations, Expenses: MSD, Merck Serono, Sanofi, Eli Lilly, Bristol-Myers Squibb

Christopher T. Harbison

Employment: Bristol-Myers Squibb

Stock or Other Ownership: Bristol-Myers Squibb

Cecile Dorange

Employment: Bristol-Myers Squibb

Stock or Other Ownership: Bristol-Myers Squibb

Marina Tschaika

Employment: Bristol-Myers Squibb

Stock or Other Ownership: Bristol-Myers Squibb

Dung T. Le

Honoraria: Merck

Consulting or Advisory Role: Merck, Bristol-Myers Squibb

Research Funding: Bristol-Myers Squibb

Patents, Royalties, Other Intellectual Property: Checkpoint blockade and microsatellite instability

Supplementary Study Design

The criteria for discontinuation of treatment included the following treatment-related adverse events: grade 2 uveitis, grade 3 nonskin events lasting ≥ 7 days, grade 3 laboratory abnormalities of thrombocytopenia or liver function, all grade 4 events, and laboratory abnormalities, except for asymptomatic amylase or lipase elevations. The criteria for dose delay (until resolution of the treatment-related adverse event to grade 1 or lower) of nivolumab, ipilimumab, or both include the following treatment-related adverse events: grade 2 or worse nonskin events (except for grade 2 fatigue or laboratory abnormalities, which do not require a treatment delay), grade 3 skin events, and grade 3 laboratory abnormalities (except for asymptomatic amylase and lipase increases). If the patient had normal AST, ALT, or total bilirubin concentrations at baseline, the dose would be delayed for grade 2 or worse adverse events; if these laboratory parameters were grade 1 at baseline, the dose would be delayed for grade 3 or worse adverse events.


Table A1. Best Overall Response per Blinded Independent Central Review by PD-L1 Status


We thank the patients and their families, investigators, and research staff at all study sites; Ono Pharmaceutical, Osaka, Japan, and the staff of Dako North America for collaborative development of the automated programmed death-ligand 1 immunohistochemical assay; and Olaf Christensen and Marsha Smith for their support. Editorial assistance was provided by Kathy Covino of Chrysalis Medical Communications, Hamilton, NJ, and was funded by Bristol-Myers Squibb.

1. International Agency for Research on Cancer: Stomach cancer: GLOBOCAN 2012: Estimated incidence, mortality and prevalence worldwide in 2012. Google Scholar
2. International Agency for Research on Cancer: Oesophageal cancer: GLOBOCAN 2012: Estimated incidence, mortality and prevalence. Google Scholar
3. Anandappa G, Chau I: Emerging novel therapeutic agents in the treatment of patients with gastroesophageal and gastric adenocarcinoma. Hematol Oncol Clin North Am 31:529-544, 2017 Crossref, MedlineGoogle Scholar
4. Wagner AD, Syn NL, Moehler M, et al: Chemotherapy for advanced gastric cancer. Cochrane Database Syst Rev 8:CD004064, 2017 MedlineGoogle Scholar
5. Janjigian YY, Sanchez-Vega F, Jonsson P, et al: Genetic predictors of response to systemic therapy in esophagogastric cancer. Cancer Discov 8:49-58, 2018 Crossref, MedlineGoogle Scholar
6. Cancer Genome Atlas Research Network: Comprehensive molecular characterization of gastric adenocarcinoma. Nature 513:202-209, 2014 Crossref, MedlineGoogle Scholar
7. Cancer Genome Atlas Research Network: Integrated genomic characterization of oesophageal carcinoma. Nature 541:169-175, 2017 Crossref, MedlineGoogle Scholar
8. Zhang M, Dong Y, Liu H, et al: The clinicopathological and prognostic significance of PD-L1 expression in gastric cancer: A meta-analysis of 10 studies with 1,901 patients. Sci Rep 6:37933, 2016 Crossref, MedlineGoogle Scholar
9. Muro K, Chung HC, Shankaran V, et al: Pembrolizumab for patients with PD-L1-positive advanced gastric cancer (KEYNOTE-012): A multicentre, open-label, phase 1b trial. Lancet Oncol 17:717-726, 2016 Crossref, MedlineGoogle Scholar
10. Fuchs CS, Doi T, Jang RW, et al: Safety and efficacy of pembrolizumab monotherapy in patients with previously treated advanced gastric and gastroesophageal junction cancer: Phase 2 clinical KEYNOTE-059 trial. JAMA Oncol 4:e180013; 2018 Crossref, MedlineGoogle Scholar
11. Janjigian Y, Bendell JC, Calvo E, et al: CheckMate-032: Phase I/II, open-label study of safety and activity of nivolumab (NIVO) alone or with ipilimumab (IPI) in advanced and metastatic (A/M) gastric cancer (GC). J Clin Oncol 34, 2016 (suppl; abstr 4010) Google Scholar
12. Kang YK, Boku N, Satoh T, et al: Nivolumab in patients with advanced gastric or gastro-oesophageal junction cancer refractory to, or intolerant of, at least two previous chemotherapy regimens (ONO-4538-12, ATTRACTION-2): A randomised, double-blind, placebo-controlled, phase 3 trial. Lancet 390:2461-2471, 2017 Crossref, MedlineGoogle Scholar
13. Curran MA, Montalvo W, Yagita H, et al: PD-1 and CTLA-4 combination blockade expands infiltrating T cells and reduces regulatory T and myeloid cells within B16 melanoma tumors. Proc Natl Acad Sci USA 107:4275-4280, 2010 Crossref, MedlineGoogle Scholar
14. Selby M, Englehardt J, Lu L-S, et al: Antitumor activity of concurrent blockade of immune checkpoint molecules CTLA-4 and PD-1 in preclinical models. J Clin Oncol 31, 2013 (suppl; abstr 3061) Google Scholar
15. Larkin J, Chiarion-Sileni V, Gonzalez R, et al: Combined nivolumab and ipilimumab or monotherapy in untreated melanoma. N Engl J Med 373:23-34, 2015 Crossref, MedlineGoogle Scholar
16. Antonia SJ, López-Martin JA, Bendell J, et al: Nivolumab alone and nivolumab plus ipilimumab in recurrent small-cell lung cancer (CheckMate 032): A multicentre, open-label, phase 1/2 trial. Lancet Oncol 17:883-895, 2016 Crossref, MedlineGoogle Scholar
17. Overman MJ, Lonardi S, Wong KYM, et al: Durable clinical benefit with nivolumab plus ipilimumab in DNA mismatch repair-deficient/microsatellite instability-high metastatic colorectal cancer. J Clin Oncol 36:773-779, 2018 LinkGoogle Scholar
18. Eisenhauer EA, Therasse P, Bogaerts J, et al: New response evaluation criteria in solid tumours: Revised RECIST guideline (version 1.1). Eur J Cancer 45:228-247, 2009 Crossref, MedlineGoogle Scholar
19. National Cancer Institute: NCI Common Terminology Criteria for Adverse Events (CTCAE) v4.0. Bethesda, MD, National Cancer Institute, 2009 Google Scholar
20. Umar A, Boland CR, Terdiman JP, et al: Revised Bethesda guidelines for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and microsatellite instability. J Natl Cancer Inst 96:261-268, 2004 Crossref, MedlineGoogle Scholar
21. Simon R: Optimal two-stage designs for phase II clinical trials. Control Clin Trials 10:1-10, 1989 Crossref, MedlineGoogle Scholar
22. Wolchok JD, Kluger H, Callahan MK, et al: Nivolumab plus ipilimumab in advanced melanoma. N Engl J Med 369:122-133, 2013 Crossref, MedlineGoogle Scholar
23. Hammers HJ, Plimack ER, Infante JR, et al: Safety and efficacy of nivolumab in combination with ipilimumab in metastatic renal cell carcinoma: The CheckMate 016 Study. J Clin Oncol 35:3851-3858, 2017 LinkGoogle Scholar
24. Hellmann MD, Rizvi NA, Goldman JW, et al: Nivolumab plus ipilimumab as first-line treatment for advanced non-small-cell lung cancer (CheckMate 012): Results of an open-label, phase 1, multicohort study. Lancet Oncol 18:31-41, 2017 Crossref, MedlineGoogle Scholar
25. Muro K, Fuchs CS, Jang RW, et al: KEYNOTE-059 cohort 1: Pembrolizumab (Pembro) monotherapy in previously treated advanced gastric or gastroesophageal junction (G/GEJ) cancer in patients (Pts) with PD-L1+ tumors: Asian subgroup analysis. J Clin Oncol 36, 2018 (suppl, abstr 723) Google Scholar
26. Opdivo (nivolumab) US prescribing information: Princeton, NJ, Bristol-Myers Squibb, 2018 Google Scholar
27. Le DT, Durham JN, Smith KN, et al: Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade. Science 357:409-413, 2017 Crossref, MedlineGoogle Scholar
28. Le DT, Uram JN, Wang H, et al: PD-1 blockade in tumors with mismatch-repair deficiency. N Engl J Med 372:2509-2520, 2015 Crossref, MedlineGoogle Scholar




DOI: 10.1200/JCO.2017.76.6212 Journal of Clinical Oncology 36, no. 28 (October 01, 2018) 2836-2844.

Published online August 15, 2018.

PMID: 30110194

ASCO Career Center